TY - CHAP
T1 - Segmentation of the comet assay images
AU - Smolka, Bogdan
AU - Lukac, Rastislav
PY - 2004
Y1 - 2004
N2 - The single cell gel electrophoresis, called Comet Assay, is a microelectrophoretic technique of direct visualization of the DNA damage at the cell level. In the comet assay, the cells suspended in an agarose gel on a microscope slide are subjected to lysis, unwinding of DNA and electrophoresis. Under the influence of weak, static electric field, charged DNA migrates away from the nucleus, forming the so called comet. The damage is quantified by measuring the amount of the genetic material, which migrates from the nucleus to form the comet tail. In this paper we present three novel methods of the comet tail and head extraction, which allow to quantify the cell's damage.
AB - The single cell gel electrophoresis, called Comet Assay, is a microelectrophoretic technique of direct visualization of the DNA damage at the cell level. In the comet assay, the cells suspended in an agarose gel on a microscope slide are subjected to lysis, unwinding of DNA and electrophoresis. Under the influence of weak, static electric field, charged DNA migrates away from the nucleus, forming the so called comet. The damage is quantified by measuring the amount of the genetic material, which migrates from the nucleus to form the comet tail. In this paper we present three novel methods of the comet tail and head extraction, which allow to quantify the cell's damage.
UR - https://www.scopus.com/pages/publications/35048875319
U2 - 10.1007/978-3-540-30126-4_16
DO - 10.1007/978-3-540-30126-4_16
M3 - Chapter
AN - SCOPUS:35048875319
SN - 3540232400
SN - 9783540232407
T3 - Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
SP - 124
EP - 131
BT - Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
A2 - Campilho, Aurelio
A2 - Kamel, Mohamed
PB - Springer Verlag
ER -