TY - GEN
T1 - Engineering of efficient biocatalysts using nanostructured mesoporous silicate carriers
AU - Szymańska, Katarzyna
AU - Bryjak, Jolanta
AU - Jarzȩbski, Andrzej B.
PY - 2008
Y1 - 2008
N2 - Comparative studies of the biocatalysts with invertase immobilized on three nanostructured silicate carriers: mesoporous cellular foams and two types of SBA-15 materials using three highly recommended protein bonding methods are reported. Invertase was attached to the surface by: physical adsorption followed by its crosslinking, adsorption on aminated MS followed by its crosslinking, and covalent bonding with aminated MS via GLA spacer. Experiments clearly indicate that activity of the aminated MCF-based biocatalysts is significantly higher than of both SBA-15-based counterparts. The microenvironment of proteins and porous texture of supports appear the most important factors for enzyme activity expression, and in this respect the unique properties of aminated MCFs can hardly be matched.
AB - Comparative studies of the biocatalysts with invertase immobilized on three nanostructured silicate carriers: mesoporous cellular foams and two types of SBA-15 materials using three highly recommended protein bonding methods are reported. Invertase was attached to the surface by: physical adsorption followed by its crosslinking, adsorption on aminated MS followed by its crosslinking, and covalent bonding with aminated MS via GLA spacer. Experiments clearly indicate that activity of the aminated MCF-based biocatalysts is significantly higher than of both SBA-15-based counterparts. The microenvironment of proteins and porous texture of supports appear the most important factors for enzyme activity expression, and in this respect the unique properties of aminated MCFs can hardly be matched.
KW - Enzyme immobilization
KW - Invertase
KW - Mesoporous silica
UR - https://www.scopus.com/pages/publications/52649145326
M3 - Conference contribution
AN - SCOPUS:52649145326
SN - 9781420085075
SN - 9781420085044
T3 - Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008
SP - 293
EP - 296
BT - Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008
T2 - 2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008
Y2 - 1 June 2008 through 5 June 2008
ER -