Diffuse Reflectance Infrared Fourier Transform Spectra of Biofilm-Containing Sediments: Influence of Sample Lyophilization and Drying Temperature

Monitoring of biofilms in sediments formed inside drinking water distribution systems is important due to their destructive activity and their degradation of water quality. The goal of this study was to examine the influence of drying mode and temperature upon sediments containing biofilms using diffuse reflectance infrared Fourier transform spectroscopy. Sediments were collected from sections of potable water pipes and dried at 22, 40, 105 degrees Celsius, or lyophilized. The presence of biofilm residues was demonstrated. Lyophilization of biofilms and drying at temperatures higher than 22 degrees Celsius affected the infrared spectra. The least invasive method involved air-drying of the biofilms at 22 degrees Celsius.