Bison bonasus skull from the Bihor Mountains, Romania: Isotopic and morphological investigations

In this study, we investigated morphologically and geochemically a skull from an open pit situated on the Călineasa-Şesu Gârzii Plateau, Bihor Mountains. The study presents the first 14C dating and stable isotope composition of bison skeletal remains from the Romanian Carpathians. The result of 14C determination yielded two relevant maxima: one is around AD 1550 and the second around AD 1645. The data indicate that the investigated bison died during the ‘Little Ice Age’ (LIA), approximately 200 years before the last ones were hunted to extinction. Stable isotope composition of carbon from bison tooth enamel suggests that the bison diet consisted mainly of C3 grasses, compatible with a high-altitude habitat, vegetation distribution and low mobility. Oxygen isotope composition of both phosphate and carbonate groups of tooth enamel indicates that the bison drank from stagnant water sources, such as lakes or puddles formed from rain water. This further suggests low mobility of the bison as well, as the presence of such small stagnant water sources is characteristic even today for the plateau. Calculated oxygen isotope compositions of rain and drinking water at the time the bison lived indicate a lower relative humidity, around 60–70% at that period of LIA when the bison lived, which is lower than today’s humidity of 80%.