TY - GEN
T1 - The Kinetic of Silver Ions Release from Hydroxyapatite-AgNPs
AU - Bolshanina, Svetlana
AU - Radchenko, Olexandra
AU - Yanovska, Anna
AU - Holubnycha, Viktoriia
AU - Tverezovska, Olesia
AU - Banasiuk, Rafal
AU - Husak, Yevheniia
N1 - Publisher Copyright:
© 2022 IEEE.
PY - 2022
Y1 - 2022
N2 - The difficulties in quantitative analysis of Ag+ ions are caused by their joined presence with AgNPs. In this work silver ions release from AgNPs and HA-AgNPs were investigated. The effect of ultrasound treatment on Ag+ ions release was estimated. The Ag nanoparticles with cubic shape and size from 80 to 800 nm were used for experiments. The incubation of AgNPs demonstrates slow Ag+ release that does not exceed 5μg/mL per 24 hours but ultrasound treatment increased ion release that could be used in the combined treatment of infected wounds. Hydroxyapatite (HA) obtained by three various syntheses was mixed with AgNPs to study Ag+ ions released in the presence of HA after ultrasound treatment. Centrifugation was used for separation of Ag+ containing supernatant solution from AgNPs precipitate. The amount of Ag+ ions was estimated in supernatant solution after centrifugation. The ability of HA to decrease the release of Ag+ ions from AgNPs due to adsorption was investigated at three kinds of hydroxyapatites that differed by the relation of components and types of synthesis. The highest Ag+ ion release ability was observed for calcium-deficient apatite which could be explained due to the vacancies in the Ca-deficient apatite crystal structure. The highest amount of released Ag+ ions was 1.2-1.3 μg/mL. Silver ions release from AgNPs into the solution was observed in the first hours as well as after 6 days of preservation. The average release was no more than 4\μg/mL per 24 hours. After pretreatment of AgNPs in an ultrasound bath silver ions release is increased. The antibacterial properties of the AgNPs-HA composites were studied for concentrations of AgNPs in calcium deficient HA equal 5,10,20,50,100,200μg/mL. The result was evaluated by measuring the zone of bacterial growth inhibition. Both supernatant (containing Ag+ ions) and precipitate (AgNPs) have antibacterial properties, but their action is mainly by contact mechanism, so precipitate effectiveness is higher. The ultrasound didn't influence nanoparticles' effectiveness but decreased the amount of, Ag+ ion in the samples.
AB - The difficulties in quantitative analysis of Ag+ ions are caused by their joined presence with AgNPs. In this work silver ions release from AgNPs and HA-AgNPs were investigated. The effect of ultrasound treatment on Ag+ ions release was estimated. The Ag nanoparticles with cubic shape and size from 80 to 800 nm were used for experiments. The incubation of AgNPs demonstrates slow Ag+ release that does not exceed 5μg/mL per 24 hours but ultrasound treatment increased ion release that could be used in the combined treatment of infected wounds. Hydroxyapatite (HA) obtained by three various syntheses was mixed with AgNPs to study Ag+ ions released in the presence of HA after ultrasound treatment. Centrifugation was used for separation of Ag+ containing supernatant solution from AgNPs precipitate. The amount of Ag+ ions was estimated in supernatant solution after centrifugation. The ability of HA to decrease the release of Ag+ ions from AgNPs due to adsorption was investigated at three kinds of hydroxyapatites that differed by the relation of components and types of synthesis. The highest Ag+ ion release ability was observed for calcium-deficient apatite which could be explained due to the vacancies in the Ca-deficient apatite crystal structure. The highest amount of released Ag+ ions was 1.2-1.3 μg/mL. Silver ions release from AgNPs into the solution was observed in the first hours as well as after 6 days of preservation. The average release was no more than 4\μg/mL per 24 hours. After pretreatment of AgNPs in an ultrasound bath silver ions release is increased. The antibacterial properties of the AgNPs-HA composites were studied for concentrations of AgNPs in calcium deficient HA equal 5,10,20,50,100,200μg/mL. The result was evaluated by measuring the zone of bacterial growth inhibition. Both supernatant (containing Ag+ ions) and precipitate (AgNPs) have antibacterial properties, but their action is mainly by contact mechanism, so precipitate effectiveness is higher. The ultrasound didn't influence nanoparticles' effectiveness but decreased the amount of, Ag+ ion in the samples.
KW - AgNPs
KW - hydroxyapatite
KW - silver ions release
KW - sorption
KW - ultrasound treatment
UR - https://www.scopus.com/pages/publications/85142833796
U2 - 10.1109/NAP55339.2022.9934215
DO - 10.1109/NAP55339.2022.9934215
M3 - Conference contribution
AN - SCOPUS:85142833796
T3 - Proceedings of the 2022 IEEE 12th International Conference "Nanomaterials: Applications and Properties", NAP 2022
BT - Proceedings of the 2022 IEEE 12th International Conference "Nanomaterials
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 12th IEEE International Conference "Nanomaterials: Applications and Properties", NAP 2022
Y2 - 11 September 2022 through 16 September 2022
ER -