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Immobilisation for a stable biocatalyst application

  • Wrocław University of Science and Technology

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Citations (Scopus)

Abstract

This publication highlights the importance of immobilisation in obtaining stable biocatalysts. It is a review of the main immobilisation methods with their advantages and disadvantages. A distinction is made between methods that require a support (adsorption, covalent binding, binding via transition metals, entrapment/encapsulation) and those that do not (cross-linking, immobilisation in a reactor volume). The main characteristics that a material should have in order to be a suitable carrier for enzymes and the most popular carrier groups are discussed. The paper also highlights the factors that should be considered when designing an immobilisation methodology and the main parameters that characterise the immobilisation process. Attention is also given to the selection of a suitable reactor solution.

Original languageEnglish
Title of host publicationBiocatalysis Identifying novel enzymes and applying them in cell-free and whole-cell biocatalysis
EditorsDirk Tischler
PublisherAcademic Press Inc.
Pages465-488
Number of pages24
ISBN (Print)9780443317880
DOIs
Publication statusPublished - Jan 2025

Publication series

NameMethods in Enzymology
Volume714
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Enzyme carriers
  • Enzyme immobilisation
  • Immobilisation methods

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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