TY - GEN
T1 - Application of image processing algorithms in proteomics
T2 - International Conference on Computer Vision and Graphics, ICCVG 2012
AU - Jonak, Katarzyna
AU - Jedrasiak, Karol
AU - Polański, Andrzej
AU - Puszyński, Krzysztof
PY - 2012
Y1 - 2012
N2 - Studying changes in the cell after treatment by a potentially genotoxic agent is a very important approach in biological experimental techniques. A researcher in molecular biology can study the structure of cellular pathways related to responses to external agents stress, damage or ionizing radiation by measuring the amount of cellular species, such as certain proteins, RNA or DNA. For this aim the electrophoresis-based tests are widely used. In this paper we will focus on the Western blot assay for estimating the quantity of the proteins. Often such information is obtained by visual analysis of a gel, which is prejudicial and time-consuming. We developed a new, rapid and exact image processing method for automatic detection of the spots in 2-D gels and calculation the quantity of the protein produced by the cell. The proposed method can significantly reduce the time required for analysis. We have obtained very promising results with accuracy more than 96% allowing for automated analyzes of 2-D gel electrophoresis images.
AB - Studying changes in the cell after treatment by a potentially genotoxic agent is a very important approach in biological experimental techniques. A researcher in molecular biology can study the structure of cellular pathways related to responses to external agents stress, damage or ionizing radiation by measuring the amount of cellular species, such as certain proteins, RNA or DNA. For this aim the electrophoresis-based tests are widely used. In this paper we will focus on the Western blot assay for estimating the quantity of the proteins. Often such information is obtained by visual analysis of a gel, which is prejudicial and time-consuming. We developed a new, rapid and exact image processing method for automatic detection of the spots in 2-D gels and calculation the quantity of the protein produced by the cell. The proposed method can significantly reduce the time required for analysis. We have obtained very promising results with accuracy more than 96% allowing for automated analyzes of 2-D gel electrophoresis images.
UR - https://www.scopus.com/pages/publications/84868032353
U2 - 10.1007/978-3-642-33564-8_52
DO - 10.1007/978-3-642-33564-8_52
M3 - Conference contribution
AN - SCOPUS:84868032353
SN - 9783642335631
T3 - Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
SP - 433
EP - 440
BT - Computer Vision and Graphics - International Conference, ICCVG 2012, Proceedings
PB - Springer Verlag
Y2 - 24 September 2012 through 26 September 2012
ER -